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Background: |
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. |
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Applications: |
ELISA |
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Name of antibody: |
FITC |
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Immunogen: |
KLH conjugated FITC |
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Full name: |
Fluorescein isothiocyanate |
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ELISA Recommended dilution: |
5000-10000 |
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